CURRICULUM MAP: 10436.map
Biotechnology (ACP) 555/554
27.3 SCIENCE - LIFE SCIENCE (V,VI,VII)
How can manipluation of the genetic information in a cell be used to produce new traits in an organism?
How should the application of these technologies be regulated?
One of the focuses of biotechnology is the transfer of DNA from one organism to another called genetic engineering. This unit covers the many applications of this technology. Students will learn the scientific concepts behind the technology and then discuss the social and ethical implication of the application of the technology. Topics discussed are bacterial transformation, genetically modified foods, and gene therapy. Students perform a bacterial transformation and purify the recombinant protein from the transformed bacteria.
Students in Biotechnology will develop the ability to:
A. Define genetic engineering as the transfer of DNA between different organisms,
B. Demonstrate how restriction enzymes "cut" DNA at restriction sites,
C. Explain how restriction enzymes, DNA ligase, and plasmids are used to construct recombinant DNA,
D. Relate the natural use restriction enzymes to their biotechnology application,
E. Explain that plasmids are circular pieces of DNA found in bacteria,
F. Demonstrate the ability to conduct a bacterial transformation and explain the importance of each step in the process,
G. Demonstrate the application of their knowledge of E.coli and the use of sterile technique by preparing media plates and streaking plates to grow single colonies,
H. Interpret results on plates with different media (AMP/noAMP) and transformed and non-transformed cells,
I. Calculate transformation efficiency,
J. Explain that the appearance of transformed colonies is the phenotypic expression of gene transfer,
K. Explain the physical properties of molecules used to perform separation as in chromatography,
L. Conduct hydrophobic interaction column chromatography to purify the Green Fluorescent protein.
Regarding Genetic Engineering, students will develop the abiilty to:
M. Describe the mechanisms by which plants are genetically engineered,
N. Discuss specific application of Genetically Modified Organisms for agricultural use,
O. Evaluate the benefits and risks of use of genetically modified organisms for food production.
Regarding Gene Therapy, students will develop the ability to:
P. Recognize that some diseases can be treated by gene therapy but other diseases cannot be treated in this way,
Q. Using a list of criteria, determine which diseases are good candidates for gene therapy,
R. Describe ways to introduce the gene into cells for gene therapy and evaluate the benefits and risks for each method,
S. Distinguish between treatment of a genetic disease and genetic enhancement.
pGlo Bacterial Transformation Lab
GFP Purification Lab
View Nova/Frontline Special "Harvest of Fear"
Subjective and Objective Testing
Labs and Lab notebook
Genetic Science Learning Center - "Gene Therapy- A Molecular Bandage" Module
Bacterial Transformation and GFP Purification Labs Teacher Guides - BioRad
Nova/Frontline "Harvest of Fear"